2 x YT medium

Product information

 Description:
A nutritious liquid medium formulated to promote the growth of host cells, thereby encouraging increased replication and yield from
filamentous single stranded bacteriophages (such as the M13 phage).

Formula g/litre
Tryptone 16.0
Yeast extract 10.0
Sodium chloride 5.0

Method for reconstitution
Weigh 31.0 grams of powder and disperse in 1 litre of deionised water.
Allow the mixture to soak for 10 minutes, swirl to mix and sterilise
by autoclaving at 121°C for 15 minutes.

Appearance: Straw, clear liquid.

pH: 7.0 ± 0.2

Minimum Q.C. organisms: Escherichia coli DH5 (ATCC® 53868).

Storage of Prepared Medium: Capped containers – up to 3 months at 15-20°C in the dark.

Inoculation: Mix fresh overnight culture of host cells with bacteriophage and use to inoculate 2xYTBroth.

Incubation: 37°C aerobically, typically for 4-5 hours to reduce risk of selecting deletion mutants.

Interpretation: Concentrate bacterial cells by centrifugation and transfer supernatant containing bacteriophage to a fresh tube,
being careful not to disturb the pellet formed. The resulting bacteriophage stock can be stored at +4°C or -20°C